Diarylpentanoid (1,5-bis(4-hydroxy-3-methoxyphenyl)-1,4-pentadiene-3-one) (MS13) Exhibits Anti-proliferative, Apoptosis Induction and Anti-migration Properties on Androgen-independent Human Prostate Cancer by Targeting Cell Cycle–Apoptosis and PI3K Signalling Pathways

Diarylpentanoids exhibit a high degree of anti-cancer activity and stability in vitro over curcumin prostate cancer cells. Hence, this study aims to investigate the effects diarylpentanoid, 1,5-bis(4-hydroxy-3-methoxyphenyl)-1,4-pentadiene-3-one (MS13) on cytotoxicity, anti-proliferative, apoptosis-inducing, anti-migration properties, underlying molecular mechanisms treated androgen-independent cells, DU 145 PC-3. A cell viability assay has shown greater cytotoxicity MS13-treated cells (EC 50 7.57 ± 0.2 µM) PC-3 7.80 0.7 compared : 145; 34.25 2.7 µM PC-3; 27.77 6.4 µM). In addition, MS13 exhibited significant anti-proliferative against AIPC dose- time-dependent manner. Morphological observation, increased caspase-3 activity, reduced Bcl-2 protein levels these indicated that induces apoptosis time- dose-dependent. Moreover, effectively inhibited migration Our results suggest cycle-apoptosis PI3K pathways were topmost impacted by activity. findings may demonstrate modulating DEGs associated with pathways, thus inhibiting proliferation as well inducing